A pair of primers were designed according to the published sequence of gnrh2 ' s gene mrna and transporter gene with oligo version 4 . 1 softwarre , they were annealed by their 3 ' terminal brief complementary base sequence to form small segment double chain , therery , they serve mutually as template and primer , and their extension were carried out to synthesize 90 bp " gnrh / trs 本研究根據(jù)genbank中已發(fā)表的人gnrh2基因mrna序列以及轉(zhuǎn)運(yùn)肽( transporter , trs )基因核苷酸序列,借助oligo4 . 1設(shè)計(jì)了一對(duì)寡核苷酸引物,以引物3末端的短互補(bǔ)序列退火形成小段雙鏈,從而互為模板和引物,通過(guò)引導(dǎo)合成長(zhǎng)達(dá)90bp的gnrh trs序列g(shù)nrh trs 。